x

75% of people globally want unbiased news (Pew Research) but are concerned they're not getting that.

Get balanced coverage with Symby. See how stories are covered across diverse outlets and spot when one side covers a story the other skips.

PODCAST

Listen In - Bitesize Bio Webinar Audios

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you"ll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the

science life sciences

All Episodes

48:31

Discovering PARP inhibitor resistance with CRISPR

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse and breast cancer cell lines, Dr. Pettitt’s team developed a targeted, genome-wide mutagenesis screen to identify mutations responsible for resistance to the potent PARP inhibitor talazoparib (BMN 673). The screen yielded one particularly interesting point mutation in the PARP1 gene. This mutation disrupted the ability of PARP1

en

67:26

14 Ways to Measure Immune Cell Activation

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

We’ll discuss specific assays in each of these categories, the joys and pitfalls of each assay, and recommendations on how to choose the best method. You will learn tips and strategies for successful assay development using the following methods:Proliferation:3H-Thymidine UptakeBromodeoxyuridine Uptake (BrdU)ATP LuminescenceFluorescent Dye Reduction (CFSE)Cytokine Measurement:Multiplex vs. Single CytokineChoice of Cytokine (IFNg, TNFa, IL-6, IL-1?, etc.)Kinetics of Cytokine ReleaseSurface

en

70:56

A Guide to Target Gene Validation With Quantitative...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

While next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of publication. In this webinar, you will learn:• Low (1-5 genes) vs medium (~300 genes) throughput experimental design• Pros and cons of self-designed vs “off the shelf” assays• How to set up your wet lab experiments start to finish• Downloadable example

en

46:38

How to Ensure Your Cell-Based Assays Are Reproducible

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Join us in this webinar featuring Dr. Vicki Doronina as she takes you through vital components of assay design.In this webinar, you will learn:How to choose between immortalized cells and primary cells for your assayHow to avoid sources of bias in your cell-based assaysHow to use high throughput assays, so you can achieve greater reproducibilityBy now, you have heard about the reproducibility crisis—the inability of scientists to reproduce experimental results. This crisis spurred journals

en

67:21

Simultaneous Proteomics and Transcriptomics

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Join us in this webinar featuring Dr. Marlon Stoeckius as he explains how you can improve your single-cell RNA-sequencing (scRNA-seq) experiments.In this tutorial, you will find:-How you can run one scRNA-seq experiment with numerous protein markers in parallel-How you can increase your recovery of single cells (up to four times!) per experiment-How you can link phenotypes to transcriptomic profiles—with higher throughput methods!The last few years have seen the scale of single cell RNA-seq

en

58:08

Using qPCR to Validate Epigenetic Enrichment of...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

qPCR is one of the most specific and sensitive tools in molecular biology, allowing the quantification of target DNA molecules present at less than 1 in 106. Next Generation Sequencing (NGS) has similar potential. However, the presence of large amounts of non-target DNA in most clinical or environmental samples precludes easy and inexpensive analysis of rare events.In this webinar, you will learn:1. The epigenetic differences between microbial and human/animal genomes2. The use of

en

33:47

Super-Resolved STED Spectroscopy: New insights

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive observation of the interactions in the living cell is often impeded by principle limitations of conventional far-field optical microscopes, for example with respect to limited spatio-temporal resolution. We

en

56:11

Challenges and Solutions in Microbiome Analysis...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Elucidating meaningful, unbiased microbial community profiles from complex microbiome samples is challenging. In this webinar, you will learn:– the sources of bias throughout the microbiome analysis workflow– practical solutions for troubleshooting your techniques– new technologies to achieve the most representative and unbiased microbiome profilesJoin Dr. Sven Reister as he guides you through a typical workflow for analysis of microbial community profiles of complex and low biomass

en

55:34

Precise and Fast Spectral FLIM at Video Rate in Confocal

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the ratio of the donor and acceptor intensities (sensitized emission) or by detecting the excited state lifetime of the donor, which decreases with increasing FRET (Fluorescence Lifetime IMaging or FLIM). FLIM is robust, immune to bleaching and inherently quantitative. On confocal

en

87:30

Complete and Fast 3D Image Analysis in Microscopy

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

If image analysis is a place you fear to tread, or if you struggle with over complicated and time-consuming microscopy image analysis workflows, this is your opportunity to go beyond those limits.You will learn a fast, efficient and flexible approach to 4D microscopy image analysis, which yields high quality images and results.We’ll cover:• How to handle and process large image data quickly• How to detect cells, nuclei, membranes and cellular structures easily with interactive image

en

55:16

A Guide to Harnessing DNA Assembly for Drug Discovery

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Current DNA synthesis and assembly technologies give today’s genetic engineers unprecedented freedom to control every aspect of genetic design. In this webinar on DNA assembly, you will learn:Key concepts in DNA assemblyThe importance of analyzing combinatorial libraries of genetic designs for natural product biosynthesisEmerging areas of researchJoin Dr. Michael Smanski as he discusses his group’s work in engineering natural products, specialized metabolites that are not necessary for

en

35:06

Get the Best Microscopy Images with Huygens Batch...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, you will learn about the advantages of deconvolving all your images fully automatically. Discussed in this webinar:– How to improve the quality of your images with the new Batch Express– How to increase resolution and signal of all your fluorescent microscopy data– How to deconvolve your new acquired images instantly– If you are interested in improving your fluorescent microscopy images, without manual intervention, then this is the webinar for you!The Huygens software is

en

39:24

Long Adapter Single Stranded Oligonucleotide (LASSO)...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn:The difference and benefits of LASSO cloning over NGSHow LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbiome genomesHow this technology can impact our understanding of disease, drug discovery, precision medicine, and so much moreJoin Dr. Lorenzo Tosi

en

65:39

2D Superresolution mode for ZEISS Airyscan – Fast and...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Utilizing a pinhole-plane imaging concept, ZEISS Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as a very small pinhole to increase resolution while the overall detector design delivers better signal-to-noise than traditional GaAsP-based confocal systems. In the past, a stack of at least five z-slices had to be deconvolved to get

en

62:20

Designing Flanking Homology DNA Assembly Experiments

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Join us in this webinar on demystifying DNA assembly. In this webinar, you will learn:– How flanking homology DNA assembly methods work– How to use web-based software to design experimental methods for flanking homology DNA assembly methods– How synthetic DNA fragments fit in to the DNA assembly processDr. Nathan Hillson will discuss methods in flanking homology DNA assembly, including Gibson, In-Fusion, and yeast TAR assembly—amongst many other related methods. Current DNA assembly methods

en

62:43

Biological Applications of X-Ray Microscopy and...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, you will learn about the power of 3D imaging of larger samples. In particular, you will:Gain insight in to how to use x-ray microscopy to analyze biological samplesLearn the basics about sample preparation for x-ray microscopyUnderstand the benefits and drawbacks to different imaging conditionsX-Ray Microscopy (XRM) is a relatively new technique that combines the geometric magnification of traditional micro-CT with the optical magnification of light microscopy. Using XRM

en

67:21

Large Volume Serial Blockface Electron Microscopy...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

An unlikely renaissance is occurring in biological imaging, propelled by the ongoing development of serial blockface electron microscopy imaging technologies. Imaging systems based on scanning EM instruments place large-volume automated EM serial imaging within reach of most labs. How best to take advantage of the automation and versatility? Large scale connectomics studies are stunning in their scope and detail. Smaller scale SBFI applications, however, offer academic labs and preclinical

en

53:42

Optocardiography: Optical Imaging of Cardiac Physiology

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, you will learn how to use optical imaging for functional cardiac physiological mapping of transmembrane potential, calcium handling, and metabolism.-The main aspects covered in the webinar include:-How to design an effective optocardiography system and select optimal fluorescence probes for your research needs using open source hardware and software.-Troubleshooting tips for reducing noise and motion artifacts during data acquisition-Advanced optocardiography:

en

44:06

How to Conduct Localized Proteomics of Micro Regions

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this tutorial, you will learn:How to perform isolated proteomics of microscopic regions of interestHow to use this technique with FFPE tissueHow proteomics can help you understand molecular mechanisms in diseaseWe recently developed a technique that allows localized proteomics of microscopic regions of interest, such as specific cell types or neuropathological features of disease. This technique uses laser capture microdissection (LCM) to isolate regions/cells of interest followed by

en

55:55

Harnessing the Power of Heterogeneity in cryo-EM

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

A major impediment in the determination of high resolution protein structures by single particle cryo-electron microscopy has been the presence of sample heterogeneity. Oftentimes, heterogeneity is due the dynamic nature of protein complexes, which can exist in multiple different conformational states in solution. Recent advancements in cryo-electron image processing have provided tools to accurately sort vitrified protein complexes into distinct populations that can be subsequently used to

en

145 results

Similar Podcasts

Classic Radio Science Fiction

solgoodmedia.com

Mission to Mars

Inception Point Ai

The 365 Days of Astronomy

365DaysOfAstronomy.org

Creek Devil

william Jevning

UFO Chronicles Podcast

Nik Hunter