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PODCAST

Listen In - Bitesize Bio Webinar Audios

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you"ll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the

science life sciences

All Episodes

62:33

What is science writing? A guide for researchers...

Listen In - Bitesize Bio Webinar Audios ·

2023/03/30

Are you a scientist looking for a change of pace? Have you ever considered using your scientific knowledge and communication skills to become a science writer or communicator? Well, look no further! In this exciting episode of Listen In, we take you on a journey exploring the many different avenues of science writing—from technical medical writing to fun and relaxed blog and journalism styles. We'll also give you the inside scoop on how to break into the field, where to find job

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62:18

5 Principles for Creating a Rewarding Career Inside...

Listen In - Bitesize Bio Webinar Audios ·

2023/03/23

Only a tiny percentage of bioscience PhD students will progress to a PI position. This statistic means that ~95% of us need to find a career path other than the traditional academic one most of us aim for when we set out as scientists.Listen in to hear from Bitesize Bio's founder, Dr. Nick Oswald, and discover five simple but powerful principles you can use to carve out the career that best suits, excites, and rewards you inside or outside of the lab.You'll discover:• How to decide if the

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50:54

Precise, high-efficiency editing of stem cells to...

Listen In - Bitesize Bio Webinar Audios ·

2023/03/02

Discover how combining two nobel-prize winning technologies—cell reprogramming and CRISPR gene editing—creates a powerful platform to study basic cell biology, development, and disease. You'll learn how this technology overcomes problems with more traditional immortalized cell models and see how it is already being used to study neurodegenerative diseases. This webinar is presented by Bill Skarnes, Professor of Cellular Engineering at The Jackson Laboratory.Follow Bill and The Jackson

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68:22

Base editing in practice

Listen In - Bitesize Bio Webinar Audios ·

2023/02/23

Base editing allows researchers to make precise changes to the genome. Discover how this technique works, how it differs from traditional genome editing, and how it is being used to understand human variation. This webinar is presented by Alexis Komor, Assistant Professor at The University of California, San Diego. Follow Alexis and The University of California, San Diego on Twitter. Click here to view the full presentation of this webinar. Browse all episodes of the Listen In Series here:

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22:53

CRISPRi as an alternative to CRISPR-cut in human iPSCs

Listen In - Bitesize Bio Webinar Audios ·

2023/02/16

Get help and advice with designing and using CRISPRi in human iPSCs. Discover that CRISPRi is an efficient way to silence transcription, sometimes in a complete manner, with loss of H3K4me3 activation marks. Learn how to eliminate side effects of clonal expansion and sites of viral vector integration by using a method that avoids single-cell selection. You'll hear considerations of the challenges of the methods, such as target-specific lentiviral silencing, efficient silencing levels, and

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60:10

The 4 D’s of CRISPR gene editing: Design, Develop,...

Listen In - Bitesize Bio Webinar Audios ·

2023/02/09

Whether you are just getting started with CRISPR or want to refresh the basics in designing and performing CRISPR experiments, Dr. Antony Adamson can help. [1] As Manager of the Genome Editing Unit at the University of Manchester, [2] he's helped numerous researchers achieve their varied CRISPR goals. Tap into his knowledge and wisdom in this enlightening presentation; it could just be the advice you need to increase the effectiveness of your CRISPR experiments.View the full presentation of

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60:43

The World of Macrophages: How to Harness Them for...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/05

Although macrophages were first described by Elie Metchnikoff in 1882, plenty of mysteries are still associated with the cell type. Indeed, while macrophages were once considered simply “garbage trucks” of the immune system due to their phagocytic property, their substantial and multifaceted contribution to immunological responses and homeostasis is becoming more apparent. Macrophages can produce a wide range of cytokines and chemokines to influence the immune response toward healing or

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51:28

Basics of FLIM and Applications to FRET Biosensors

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Functional imaging is a rapidly growing field key to driving new understanding in biology. Insights into the function and interaction of molecules are the key to reveal the underlying cellular mechanisms. In this context, fluorescence lifetime imaging (FLIM) is a powerful tool, providing valuable information beyond spectral imaging. FLIM is immune to concentration artefacts and sensitive to molecular environment such has pH changes, ion concentrations, and more.Förster Resonance Energy

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49:31

Adding Multiplex Factor to Confocal Imaging with...

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this tutorial on confocal imaging, you will learn how you can:– Capture weaker signals—and still get sound, reproducible data.– Reach faster volumetric imaging without sacrificing resolution– Increase data throughput for your imaging needsLearn about the new Multiplex mode for parallel pixel acquisition with the ZEISS LSM 9 family and Airyscan 2. You can now acquire up to 8 superresolution image lines with high signal-to-noise rate in a single sweep. Capture dynamic processes, cellular

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60:14

Cryo-electron Tomography for Cell Biology

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, you will learn:– The complete workflow for in situ cryo-electron tomography– How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae)– How mapping these structures back into the native cellular environment reveals new molecular interactions that are only accessible by this technique (e.g., the binding of cargo to COPI-coated Golgi membranes and the tethering of proteasomes

en

59:46

Cryo-Electron Microscopy and the Complexity of Cancer

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, you will learn:– How structural biology can change the face of your research– The biological challenges of cancer research– Advantages of cryo-EM for your researchCovering both biology and methodology, this webinar will explain how single-particle cryo-electron microscopy enables us to gain insight into cancer development through the detailed analysis of molecular structure.In single-particle cryo-EM, hundreds of thousands of images formed by electron scattering of

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57:26

Fast superresolution microscopy – with Lattice SIM

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and many different labels.In this webinar, we will explain how Elyra 7 with Lattice SIM takes you beyond the diffraction limit of conventional microscopy to image your samples with superresolution. Learn how to examine the fastest processes in living samples – in

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62:18

Array Tomography for SEM 3D Reconstruction

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement.In this webinar you will get tips and tricks to optimize the workflow of your array tomography:– Fast and precise trimming of the sample block-face– Adhesion of a single section to create ribbons– Automated serial sectioning with the ARTOS 3D ultramicrotome– Acquisition and processing

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58:56

Expanding the limits of EM sample prep with ICE

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar you will learn:– Improved vitrification: Specimen vitrification without synchronisation fluid– Vitrification strategies: Optimized freezing for different techniques– Light and electrical stimulation: Dissect cellular processes with millisecond precision– A look into the future: freezing of crystalsPlunge freezing and cryo imaging of proteins and complexes have revealed new details in understanding the machinery of the cell and how molecules are involved in cellular

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39:07

Using CRISPR/Cas9 to detect DNA sequences with AFM

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Join Dr. Jason Reed as he describes a novel method by which endonuclease-inhibited Cas9 can be employed as a programmable biomarker in high-speed atomic force microscopy (HS-AFM) imaging.In this webinar, you will learn:1. How CRISPR/Cas9 can be used to “flag” alterations and mutations in DNA, rather than cut it2. How pairing atomic force microscopy (AFM) with optical equipment found in DVD players can be used to map DNA at a faster rate than traditional DNA sequencing3. Applications of this

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71:34

How to Use CRISPR to Accelerate Cancer Therapies

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Join Theo Roth as he describes his lab’s novel CRISPR-Cas9 genome-targeting system that does not require viral vectors to modify T cell genomes, but instead focuses on HDR. This allows rapid and efficient insertion of large DNA sequences at specific sites in the genomes of primary human T cells, and permits individual or multiplexed modification of endogenous genes. Importantly, avoiding the use of viral vectors will result in accelerated research and clinical applications, reduce

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52:54

Nucleic acids 101: Confirming Their Quality

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Join us in this webinar as Dr. Victoria Doronina helps you determine the quality of your nucleic acids. In this webinar you will learn:– How to choose the best method to extract your nucleic acids– Which method you should chose to determine nucleic acid quality– How to avoid common pitfalls for both extraction and quality controlVirtually all experiments in the molecular biology lab require high quality, pure nucleic acids as a starting material. This sounds simple enough. However, as

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53:01

Live Cell Isolation by Laser Microdissection

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, you will learn:– How to best prepare your specimen for live cell isolation by laser microdissection– How to optimize your laser microdissection workflow– How to avoid common pitfalls of this techniqueLaser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections, laser microdissection may be applied for live cell isolation. Unlike other

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39:57

Reliable quantification of fluorescence images

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

In this webinar, you will learn:– How to overcome issues with stage drift and crosstalk between channels– How to obtain reliable images and reproducible quantification results– How to proceed with advanced image analysis after restoring imagesDuring fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of experimental

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49:35

Crash Course: Developing a Fool-Proof ELISA

Listen In - Bitesize Bio Webinar Audios ·

2022/09/01

Join us in this webinar featuring Dr. Omonse Talton who will guide you through developing a “fool proof” enzyme-linked immunosorbent assay (ELISA). In this webinar, you will learn:– When and why you should use the different types of ELISAs– direct, indirect, sandwich, competitive/inhibition ELISA– Major considerations for developing your ELISA– Tried and tested tips for you to perform a successful ELISAThe ELISA is one of (if not the most) common techniques in biology and biochemistry

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145 results

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