PODCAST

Life sciences knowledge center

Echo Han

science life sciences

All Episodes

00:13:57

IVTScrip™ mRNA-β gal, 5-Methoxy-U modified (Cap 1, 30...

Life sciences knowledge center ·

2022/09/27

This mRNA product will express β-galactosidase (β-gal), a protein product of the baterial LacZ gene, which is a commonly used reporter gene in the mammalian systems. Furthermore, the product was polyadenylated and substituted with 5-methoxyuridine to increase its stability and translation efficiency. https://mrna.creative-biolabs.com/ivtscrip-mrna-gal-5-methoxy-u-modified-cap-1-30-nt-poly-a-10.htm

en-us

00:13:57

IVTScrip™ mRNA-β gal (Cap 1, 30 nt-poly(A))

Life sciences knowledge center ·

2022/09/27

This mRNA product will express β-galactosidase (β-gal), a protein product of the baterial LacZ gene, which is a commonly used reporter gene in the mammalian systems. https://mrna.creative-biolabs.com/ivtscrip-mrna-gal-cap-1-30-nt-poly-a-9.htm

en-us

00:13:57

IVTScrip™ mRNA-RLuc, 5-Methoxy-U modified (Cap 1, 30...

Life sciences knowledge center ·

2022/09/27

This mRNA product will express a renilla luciferase protein, which is a commonly used reporter gene in the mammalian systems. Furthermore, the product was polyadenylated and substituted with 5-methoxyuridine to increase its stability and translation efficiency. https://mrna.creative-biolabs.com/ivtscrip-mrna-rluc-5-methoxy-u-modified-cap-1-30-nt-poly-a-8.htm

en-us

00:09:29

IVTScrip™ mRNA-mCherry, 5-Methoxy-U modified (Cap 1,...

Life sciences knowledge center ·

2022/09/27

This mRNA product will express a monomeric red fluorescent protein, which is a commonly used reporter gene in the mammalian systems. Furthermore, the product was polyadenylated and substituted with 5-methoxyuridine to increase its stability and translation efficiency. https://mrna.creative-biolabs.com/ivtscrip-mrna-mcherry-5-methoxy-u-modified-cap-1-30-nt-poly-a-7.htm

en-us

00:10:28

IVTScrip™ mRNA-Fluc, 5-Methoxy-U, Cyanine 5-U...

Life sciences knowledge center ·

2022/09/27

This mRNA product will express a firefly luciferase protein, which is a commonly used reporter gene in the mammalian systems. Furthermore, the product was polyadenylated and substituted with 5-methoxyuridine and cyanine 5-U to increase its stability and translation efficiency. https://mrna.creative-biolabs.com/ivtscrip-mrna-fluc-5-methoxy-u-cyanine-5-u-modified-cap-1-30-nt-poly-a-6.htm

en-us

00:13:57

IVTScrip™ mRNA-Fluc, 5-Methoxy-U modified (Cap 1, 30...

Life sciences knowledge center ·

2022/09/27

This mRNA product will express a firefly luciferase protein, which is a commonly used reporter gene in the mammalian systems. Furthermore, the product was polyadenylated and substituted with 5-methoxyuridine to increase its stability and translation efficiency. https://mrna.creative-biolabs.com/ivtscrip-mrna-fluc-5-methoxy-u-modified-cap-1-30-nt-poly-a-5.htm

en-us

00:13:57

IVTScrip™ mRNA-Fluc (Cap 1, 30 nt-poly(A))

Life sciences knowledge center ·

2022/09/27

This mRNA product will express a firefly luciferase protein, which is a commonly used reporter gene in the mammalian systems. https://mrna.creative-biolabs.com/ivtscrip-mrna-fluc-cap-1-30-nt-poly-a-4.htm

en-us

00:13:57

IVTScrip™ mRNA-EGFP, 5-Methoxy-U, Cyanine 5-U...

Life sciences knowledge center ·

2022/09/27

This mRNA product will express an enhanced version of the green fluorescent protein (EGFP), which is a commonly used reporter gene in the mammalian systems. Furthermore, the product was polyadenylated and substituted with 5-methoxyuridine and cyanine 5-U to increase its stability and translation efficiency. https://mrna.creative-biolabs.com/ivtscrip-mrna-egfp-5-methoxy-u-cyanine-5-u-modified-cap-1-30-nt-poly-a-3.htm

en-us

00:13:57

IVTScrip™ mRNA-EGFP, 5-Methoxy-U modified (Cap 1, 30...

Life sciences knowledge center ·

2022/09/27

This mRNA product will express an enhanced version of the green fluorescent protein (EGFP), which is a commonly used reporter gene in the mammalian systems. Furthermore, the product was polyadenylated and substituted with 5-methoxyuridine to increase its stability and translation efficiency. https://mrna.creative-biolabs.com/ivtscrip-mrna-egfp-5-methoxy-u-modified-cap-1-30-nt-poly-a-2.htm

en-us

00:13:57

IVTScrip™ mRNA-EGFP (Cap 1, 30 nt-poly(A))

Life sciences knowledge center ·

2022/09/27

This mRNA product will express an enhanced version of the green fluorescent protein (EGFP), which is a commonly used reporter gene in the mammalian systems. https://mrna.creative-biolabs.com/ivtscrip-mrna-egfp-cap-1-30-nt-poly-a-1.htm

en-us

00:12:01

mRNA Reverse Transcriptase

Life sciences knowledge center ·

2022/08/24

The cloning of expressed genes involves the synthesis of DNA from mRNA using an enzyme called reverse transcriptase. Reverse transcriptase includes reverse transcriptase and a proprietary buffer designed for robust, reliable cDNA synthesis from a full range of rare and abundant transcripts. The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptase uses an RNA template and a short primer complementary to the 3' end of the RNA to

en-us

00:09:22

mRNA Ligase

Life sciences knowledge center ·

2022/08/24

There is an emerging appreciation of the existence of RNA repair pathways that rely on mRNA ligases to maintain or manipulate mRNA structure in response to purposeful mRNA breakage events. mRNA ligase is RNA repair enzymes capable of sealing breaks. The mRNA ligase can be served for joining mRNA to mRNA. Creative Biolabs offers the most extensive selection of high-quality and performance-optimized RNA ligases and masters mixes to streamline your experiments. The pre-mixed, ready-to-use formulations

en-us

00:10:28

RNase & Inhibitors

Life sciences knowledge center ·

2022/08/24

RNase is an endoribonuclease that cleaves single-stranded RNA. It catalyzes the cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. This cleavage forms a 2',3'-cyclic phosphate, which is then hydrolyzed to the corresponding 3'-nucleoside phosphate.

en-us

00:09:01

mRNA Polymerase

Life sciences knowledge center ·

2022/08/24

mRNA polymerase is a DNA-dependent mRNA polymerase that is used for in vitro mRNA synthesis with strict specificity for its respective double-stranded promoters. Large quantities of RNA can be synthesized from a DNA sequence cloned downstream under the promoters due to the high processivity and transcription frequency of mRNA polymerase. RNA produced using the mRNA polymerase is suitable for many applications in research and biotechnology, including radiolabeled RNA probe preparation, mRNA for in

en-us

00:09:22

Enzymes for mRNA Research

Life sciences knowledge center ·

2022/08/24

A series of enzymes for mRNA research, including but not limited to mRNA polymerase, RNase & inhibitors, mRNA ligase, mRNA reverse transcriptase, and mRNA capping

en-us

00:10:28

ARCA-capped mRNA Synthesis Kit

Life sciences knowledge center ·

2022/08/24

Anti-reverse cap analog (ARCA)-capped mRNA synthesis kit includes all the critical reagents for in vitro transcription to generate high yields of high-quality mRNA efficiently. This kit could incorporate an ARCA into the transcript. ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated. The advantages of the ARCA-capped mRNA synthesis kit are that ultra-high-quality components and ARCA-based capping in correct orientation

en-us

00:14:30

mRNA Purification Kit

Life sciences knowledge center ·

2022/08/24

mRNA purification kits are designed to isolate intact messenger RNA from cells, tissues, or RNA preparations for downstream applications in the study of gene expression and transcriptome. The mRNA purification kit allows yield pure, high-quality mRNA. For instance, some kits feature all necessary reagents to remove contaminants almost completely, such as genomic DNA, ribosomal RNA, and nucleases. Due to the stringent procedure of mRNA purification, high mRNA purity can be assured for increased

en-us

00:13:57

mRNA Isolation Kit

Life sciences knowledge center ·

2022/08/24

The mRNA isolation kit contains all necessary reagents and enough buffers for the isolation of mRNA from total RNA samples in less than one hour. mRNA isolation kits optimize the isolation of mRNA from different sample sizes or total RNA. The mRNA isolation kit allows for efficient and reproducible isolation of high-purity mRNA and permits the manual processing of multiple samples. It prepares large yields of highly purified mRNA from mammalian cells, tissue, and total RNA. The highly purified mRNA

en-us

00:10:28

mRNA Kits

Life sciences knowledge center ·

2022/08/24

mRNA Isolation Kit mRNA Purification Kit

en-us

00:11:48

In Vitro Transcription Vector

Life sciences knowledge center ·

2022/07/24

mRNA has become an emerging class of gene therapy because of its negligible cytotoxicity and immunogenicity. So, there is an increasing demand for efficient and robust production of mRNA molecules in both pharmaceutics and research field. In vitro transcription (IVT) is an elegant way to obtain highly pure and uniform mRNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides.

en-us

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Life sciences knowledge center

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